Rat GE 4x44K v3 Microarray Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Protocol tips
do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Publication protocol

RNA samples (n = 6) from each dietary group were pooled and analyzed by using a Rat GE 4x44K v3 Microarray (Agilent). Both FeD and FeO pooled cDNA samples were individually compared with FeA in duplicate measurements. The assignment of fluorescent Cy3 or Cy5 dye to each comparison group was alternated between the duplicates. To identify differential gene expression, values of signal intensity were log2 transformed and normalized before the Student's t-test was performed for probe-specific comparisons. Genes showing a statistically significant (P<0.05) log2-transformed fold change of at least ±2 were analyzed to identify functional biological categories by using the Database for Annotation, Visualization and Integrated Discovery (DAVID) [13]. Microarray analysis was conducted at the Interdisciplinary Center for Biotechnology at the University of Florida. The microarray data discussed herein have been deposited in NCBI's Gene Expression Omnibus [14] and are accessible through GEO Series accession number GSE44699 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE44699).

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Rat GE 4x44K v3 Microarray Kit below.

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