Medium 254CF, Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.

Protocol tips
supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.

Publication protocol

The normal human epidermal melanocytes (HEMaLP, Cascade Biologists) were grown according to the
manufacturer’s instruction. The cells were cultured in
M-254 medium supplemented with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) at 37°C in 5% CO2. All experiments were performed using cells in the passages 5–7.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Medium 254CF, Kit below.

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