DMEM

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	FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

Protocol tips

FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

Publication protocol

FLS were cultured in a humidified 10% CO2 atmosphere at 37 °C using DMEM medium supplemented with 1.0 g/L glucose, 584 mg/L l-glutamine, 10% fetal bovine serum (FBS), 10 mM HEPES buffer, 10 U/mL penicillin, and 0.1 mg/mL streptomycin. The experiments were performed with cells from passage No. 5. Routine tests for mycoplasma contamination using the PCR Mycoplasma Kit (PromoCell, Heidelberg, Germany) were negative.

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