D-MEM (High Glucose) with L-Glutamine and Phenol Red

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	Cells were maintained in culture medium, DMEM (Wako, Tokyo, Japan) that was supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and 0.1 mg/ml streptomycin and 100 U/ml penicillin (Meiji Seika, Tokyo, Japan) at 37°C in a humidified atmosphere (5% CO2). the human lung adenocarcinoma epithelial cell line, A549 (European Collection of Cell Cultures, Salisbury, United Kingdom) was used to measure TGF-β1–induced decreases in E-cadherin levels, a marker of epithelial cells, in the absence or presence of a designated agent.

Protocol tips

Cells were maintained in culture medium, DMEM (Wako, Tokyo, Japan) that was supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and 0.1 mg/ml streptomycin and 100 U/ml penicillin (Meiji Seika, Tokyo, Japan) at 37°C in a humidified atmosphere (5% CO2). the human lung adenocarcinoma epithelial cell line, A549 (European Collection of Cell Cultures, Salisbury, United Kingdom) was used to measure TGF-β1–induced decreases in E-cadherin levels, a marker of epithelial cells, in the absence or presence of a designated agent.

Publication protocol

Human fetal lung fibroblasts, HFL-1 cells, and WI-38 cells were purchased from American Type Culture Collection (Manassas, VA, USA) and RIKEN Cell Bank (Tsukuba, Ibaraki, Japan), respectively. Cells were maintained in culture medium, DMEM (Wako, Tokyo, Japan) that was supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and 0.1 mg/ml streptomycin and 100 U/ml penicillin (Meiji Seika, Tokyo, Japan) at 37°C in a humidified atmosphere (5% CO2). In some experiments, the human lung adenocarcinoma epithelial cell line, A549 (European Collection of Cell Cultures, Salisbury, United Kingdom) was used to measure TGF-β1–induced decreases in E-cadherin levels, a marker of epithelial cells, in the absence or presence of a designated agent.

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