EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Supplement the medium with fetal bovine serum (FBS), and antibiotics, ascorbic acid, vascular endothelial growth factor (VEGF), human fibroblast growth factor (hFGF-B), hydrocortisone, human epidermal growth factor (hEGF), R3-IGF-1 (insulin-like growth factor), GA-1000 (gentamicin, amphotericin B), and heparin.

Protocol tips
Supplement the medium with fetal bovine serum (FBS), and antibiotics, ascorbic acid, vascular endothelial growth factor (VEGF), human fibroblast growth factor (hFGF-B), hydrocortisone, human epidermal growth factor (hEGF), R3-IGF-1 (insulin-like growth factor), GA-1000 (gentamicin, amphotericin B), and heparin.

Publication protocol

Human pulmonary artery endothelial cells (HPAEC) were obtained from Lonza Clonetics (Walkersville, MD). HPAEC were grown in EGM-2 medium (Lonza), which contains basic growth medium (EBM-2), fetal bovine serum (FBS), and antibiotics, ascorbic acid, vascular endothelial growth factor (VEGF), human fibroblast growth factor (hFGF-B), hydrocortisone, human epidermal growth factor (hEGF), R3-IGF-1 (insulin-like growth factor), GA-1000 (gentamicin, amphotericin B), and heparin. Unless otherwise stated, cells were maintained in a 37°C incubator at 5% CO2.

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Manufacturer protocol

Download the product protocol from Lonza for EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit below.

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