PrimaPure Porcine Endothelial Cells Growth Medium

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	supplement the media with 5% fetal bovine serum in humidified air with 5% CO2 at 37 °C

Protocol tips
supplement the media with 5% fetal bovine serum in humidified air with 5% CO2 at 37 °C

Publication protocol

Bovine AECs and PAECs from the same animal were purchased from Cell Applications (San Diego, CA) and maintained in PrimaPure™ Bovine Endothelial Cell Growth Medium (BECGM) (Genlantis, San Diego, CA). Cells were incubated in a humidified controlled gas flow incubator (Forma Series II water jacketed CO2 incubator, Thermo Electron Corporation, Marietta, OH) containing 5% CO2 at 37°C. Cells were grown to confluency, used between passages 6–15, and passaged using trypsin-EDTA. Confluent AECs and PAECs were incubated in a modular incubator chamber (Billups-Rothenberg, Del Mar, CA) containing 5% CO2 and one of the following: 0%, 5%, or 13% oxygen balanced with nitrogen at 37°C. Cells for 21% oxygen treatment were incubated in a humidified controlled gas flow incubator (Forma Series II water jacketed CO2 incubator; Thermo Electron Corporation, Marietta, OH) containing 5% CO2 at 37°C for the time period indicated in the respective figure legends.

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Manufacturer protocol

Download the product protocol from Cell Applications Inc for PrimaPure Porcine Endothelial Cells Growth Medium below.

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