Porcine Endothelial Cell Growth Medium

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	Incubated at 37°C in a humidified atmosphere of 95% air and 5% CO2

Protocol tips
Incubated at 37°C in a humidified atmosphere of 95% air and 5% CO2

Publication protocol

Pig coronary arterial endothelial cells (PCAECs) used in adhesion and transmigration studies were purchased from Cell Applications Inc (San Diego, Calif). The cells were cultured as a monolayer in Porcine Endothelial Cell Growth Medium (PECGM; Cell Applications Inc) at 37°C in a humidified atmosphere of 95% air and 5% CO2, and routinely split when cell density reached 90% to 100% confluence. The cells were used between passages 3 and 6.

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