EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.	Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Mecp2 promoter DNA methylation was quantified using the Sequenom EpiTYPER platform, and primers for the target region were designed using EpiDesigner (www.epidesigner.com, Sequenom). Six CpG sites were quantified in an amplicon spanning 282 bp in the promoter and the first exon (Please see Supplementary Table 1 for primer details). All EpiTYPER assays were tested using a standard curve with methylated and unmethylated DNA. Genomic DNA was isolated and 1 μg DNA was bisulfite-treated as described above using the EZ DNA methylation kit (Zymo Research).

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2 using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2 using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.