EZ DNA Methylation kit

DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Experiment
DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2
Product
EZ DNA Methylation kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Mecp2 promoter DNA methylation was quantified using the Sequenom EpiTYPER platform, and primers for the target region were designed using EpiDesigner (www.epidesigner.com, Sequenom). Six CpG sites were quantified in an amplicon spanning 282 bp in the promoter and the first exon (Please see Supplementary Table 1 for primer details). All EpiTYPER assays were tested using a standard curve with methylated and unmethylated DNA. Genomic DNA was isolated and 1 μg DNA was bisulfite-treated as described above using the EZ DNA methylation kit (Zymo Research).

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Reviews

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2 using EZ DNA Methylation kit from Zymo Research.

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Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

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Videos

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