Rat Endothelial Cell Growth Medium

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	supplemented with 10% fetal bovine serum (FBS, Invitrogen, #10082147), 100 U/mL penicillin (Invitrogen, #15140122), 100 μg/mL streptomycin (Invitrogen, #15140122), and 4 mmol/L L‐glutamine (VWR, West Chester, PA, #45000‐676)

Protocol tips
supplemented with 10% fetal bovine serum (FBS, Invitrogen, #10082147), 100 U/mL penicillin (Invitrogen, #15140122), 100 μg/mL streptomycin (Invitrogen, #15140122), and 4 mmol/L L‐glutamine (VWR, West Chester, PA, #45000‐676)

Publication protocol

Rat aortic endothelial cells (RAEC), rat aortic smooth muscle cells (RASMC), and rat aortic adventitial fibroblasts (RAAF) were obtained from Cell Applications, Inc. (San Diego, CA; Lot #2725). RAEC were maintained in Rat Endothelial Cell Growth Media (Cell Applications, #R211‐500), and RASMC and RAAF were maintained in complete medium containing 50:50 Dulbecco's Modified Eagle's Medium – Low Glucose) and Ham's F12 (Invitrogen #11885‐092) supplemented with 10% fetal bovine serum (FBS, Invitrogen, #10082147), 100 U/mL penicillin (Invitrogen, #15140122), 100 μg/mL streptomycin (Invitrogen, #15140122), and 4 mmol/L l‐glutamine (VWR, West Chester, PA, #45000‐676). All cells were incubated at 37°C in 95% air and 5% CO2. For all RAEC experiments, plate wells were pretreated with Attachment Factor Solution (Cell Applications#123‐500) for 3 h at 37°C. For all experiments, cells used were between passages three and eight.

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