Difco™ Brain Heart Infusion Agar, BD

Protocol tips

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	supplemented with either trehalose or glucose as indicated. Anhydrous tetracycline was used at 500 ng/ml to induce expression of ptsT or treA from ectopic expression vectors.

Protocol tips
supplemented with either trehalose or glucose as indicated. Anhydrous tetracycline was used at 500 ng/ml to induce expression of ptsT or treA from ectopic expression vectors.

Publication protocol

Strains were cultured in overnight in BHI media (Difco) supplemented with 0.5% w/v yeast extract. Growth assays utilized a defined minimal medium (DMM) as described previously. supplemented with either trehalose or glucose as indicated. Anhydrous tetracycline was used at 500 ng/ml to induce expression of ptsT or treA from ectopic expression vectors.

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Manufacturer protocol

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