Chromogenic Culture Media

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	CDIF media were supplied as pre-poured ready to use plates by bioMérieux (Craponne, France); each contained meat peptone (porcine) 8.0 g, taurocholate (bovine) 1.0 g, yeast extract 3.5 g, sodium chloride 6.0 g, selective mixture 0.27 g, chromogenic mixture 0.3 g, agar 13 g and purified water. TCCFA plates were supplied by PathWest Laboratory Medicine Excel Media and followed the original formulation of CCFA ( George et al., 1979 ) apart from reducing the antibiotic concentrations by 50 % (cycloserine 250 µg ml −1 and cefoxitin 8 µg ml −1) and including egg yolk at 60 ml l −1, plus the addition of 0.1 % synthetic sodium taurocholate (Sigma Cat # T4009), ρ-hydroxyphenylacetic acid at 1 g l −1 and agar at 13 g l −1. All plates were stored at 4 °C until used.

Protocol tips

CDIF media were supplied as pre-poured ready to use plates by bioMérieux (Craponne, France); each contained meat peptone (porcine) 8.0 g, taurocholate (bovine) 1.0 g, yeast extract 3.5 g, sodium chloride 6.0 g, selective mixture 0.27 g, chromogenic mixture 0.3 g, agar 13 g and purified water. TCCFA plates were supplied by PathWest Laboratory Medicine Excel Media and followed the original formulation of CCFA ( George et al., 1979 ) apart from reducing the antibiotic concentrations by 50 % (cycloserine 250 µg ml −1 and cefoxitin 8 µg ml −1) and including egg yolk at 60 ml l −1, plus the addition of 0.1 % synthetic sodium taurocholate (Sigma Cat # T4009), ρ-hydroxyphenylacetic acid at 1 g l −1 and agar at 13 g l −1. All plates were stored at 4 °C until used.

Publication protocol

CDIF media were supplied as pre-poured ready to use plates by bioMérieux (Craponne, France); each contained meat peptone (porcine) 8.0 g, taurocholate (bovine) 1.0 g, yeast extract 3.5 g, sodium chloride 6.0 g, selective mixture 0.27 g, chromogenic mixture 0.3 g, agar 13 g and purified water. TCCFA plates were supplied by PathWest Laboratory Medicine Excel Media and followed the original formulation of CCFA ( George et al., 1979 ) apart from reducing the antibiotic concentrations by 50 % (cycloserine 250 µg ml −1 and cefoxitin 8 µg ml −1) and including egg yolk at 60 ml l −1, plus the addition of 0.1 % synthetic sodium taurocholate (Sigma Cat # T4009), ρ-hydroxyphenylacetic acid at 1 g l −1 and agar at 13 g l −1. All plates were stored at 4 °C until used.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Bacterial cell culture media Clostridium difficile using Chromogenic Culture Media from Biomerieux.

Manufacturer protocol

Download the product protocol from Biomerieux for Chromogenic Culture Media below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Bacterial cell culture media Clostridium difficile using Chromogenic Culture Media from Biomerieux. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.