THIOGLYCOLLATE BROTH USP

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE. -pH 7.1 ± 0.2 @ 25°C

Protocol tips
-PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE. -pH 7.1 ± 0.2 @ 25°C

Publication protocol

One gram sample was suspended in 9 ml of sterilized distilled water and 1 ml was removed and inoculated directly on Sab agar plates at 37 °C for 24 h. Selective and nonselective culture media were used for isolation of the microbial contaminants, ie, nutrient agar (Oxoid, England), Sabouraud’s dextrose agar, thiosulfate-citrate-bile-sucrose agar, and MacConkey agar. For instance, Clostridium tetani was isolated by thioglycolate broth medium. Briefly, samples were inoculated directly into thioglycolate broth medium and incubated at 37 °C for 24 h. The isolates were purified in cooked meat medium and used for further analysis. Finally, important microbiological testes include gram stain, urease, indole, citrate utilization, arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, gelatin hydrolysis, coagulase, amylase and inositol were performed in lab to identify the genus and species of unknown bacterial. All analyses stages, including handling procedures, dilution, and culture media, were conducted in accordance with the US Pharmacopeia (USP) which are harmonized with the European Pharmacopoeia (EP).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for THIOGLYCOLLATE BROTH USP below.

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