INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics).

Protocol tips

One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics).

Publication protocol

Deidentified stool specimens from patients who provided samples for Clostridium difficile testing were used for this evaluation. One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics). At the time of this study, BEAV agar was routinely used for VRE surveillance at the Johns Hopkins Hospital. For the purposes of this study, BEAV broth was added in order to enhance VRE detection performance. The media and BEAV broths and all chromogenic media were incubated in the dark, in non-CO2, at 35 to 37°C. The identification of the enterococci from the chromogenic agars was based on the observation of appropriately colored colonies and supplemental testing (Gram stain, catalase, and l-pyrrolidonyl-β-naphthylamide enzyme [PYR]) if required, as per the manufacturers' instructions (Table 1). Vancomycin susceptibility tests were performed using Etest (AB bioMérieux, Durham, NC) for the VRE isolates recovered from each medium.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Bacterial cell culture media Enterococcus faecalis using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI from BIOMED.

Manufacturer protocol

Download the product protocol from BIOMED for INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Bacterial cell culture media Enterococcus faecalis using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI from BIOMED. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.