VRESelect Medium #63751

Protocol tips

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	One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics).

Protocol tips

One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics).

Publication protocol

Deidentified stool specimens from patients who provided samples for Clostridium difficile testing were used for this evaluation. One microliter of remnant stool was inoculated and aseptically streaked for isolation onto each of the five chromogenic media: the InTray Colorex VRE (BioMed Diagnostics, White City, OR), chromID VRE (bioMérieux, Marcy l'Étoile, France), VRESelect (Bio-Rad, Marnes-la-Coquette, France), HardyCHROM VRE (Hardy Diagnostics, Santa Maria, CA), and Spectra VRE (Remel, Lenexa, KS), along with BEAV agar (6 μg/ml vancomycin) (Enterococcosel; BD Diagnostics, Sparks, MD) and BEAV broth (6 μg/ml vancomycin) (BEAV broth; Hardy Diagnostics). At the time of this study, BEAV agar was routinely used for VRE surveillance at the Johns Hopkins Hospital. For the purposes of this study, BEAV broth was added in order to enhance VRE detection performance. The media and BEAV broths and all chromogenic media were incubated in the dark, in non-CO2, at 35 to 37°C. The identification of the enterococci from the chromogenic agars was based on the observation of appropriately colored colonies and supplemental testing (Gram stain, catalase, and l-pyrrolidonyl-β-naphthylamide enzyme [PYR]) if required, as per the manufacturers' instructions. Vancomycin susceptibility tests were performed using Etest (AB bioMérieux, Durham, NC) for the VRE isolates recovered from each medium.

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