MRS BROTH (DE MAN, ROGOSA, SHARPE)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-pH 6.2 ± 0.2 @ 25°C.

Protocol tips
-pH 6.2 ± 0.2 @ 25°C.

Publication protocol

Lactobacillus plantarum strains were cultured in MRS broth (Oxoid Ltd., Basingstoke, United Kingdom) at 37°C without shaking. Escherichia coli TOP10 cells (Invitrogen) were grown in Brain Heart Infusion (BHI, Oxoid) broth at 37°C with shaking. Erythromycin was added to a final concentration of 10 μg/mL for L. plantarum or 200 μg/mL for E. coli. For plates, liquid medium was solidified by adding 1.5% (w/v) agar.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MRS BROTH (DE MAN, ROGOSA, SHARPE) below.

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