De Man, Rogosa and Sharpe (MRS) Broth

Bacterial cell culture media Lactobacillus paracasei

Experiment
Bacterial cell culture media Lactobacillus paracasei
Product
De Man, Rogosa and Sharpe (MRS) Broth from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
-pH at 25 °C pH6.5 ± 0.2.
-Incubation Temperature 30–35 °C.

Publication protocol

All the lactobacillus strains were activated in MRS broth for 24 h at 37 °C. These strains were cultivated in MRS medium containing 0.06 % LA for 24 h at 28 °C with a shaking rate of 120 rpm. The cells were harvested by centrifugation (8000×g, 10 min), washed twice with 0.85 % NaCl, centrifuged again, and then used as the washed cells for the reactions. The cultivation was carried out in test tubes with 10 mL of MRS containing 1 mg/mL of LA, 1 mg/mL of Tween 80 and 5 % (v/v) of the washed cells for 24 h at 37 °C and 120 rpm. Only the strain showing the highest ability to produce CLA was selected for the next steps.

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Manufacturer protocol

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