De Man, Rogosa and Sharpe (MRS) Broth

Bacterial cell culture media Lactobacillus paracasei

Experiment
Bacterial cell culture media Lactobacillus paracasei
Product
De Man, Rogosa and Sharpe (MRS) Broth from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
-pH at 25 °C pH6.5 ± 0.2.
-Incubation Temperature 30–35 °C.

Publication protocol

All the lactobacillus strains were activated in MRS broth for 24 h at 37 °C. These strains were cultivated in MRS medium containing 0.06 % LA for 24 h at 28 °C with a shaking rate of 120 rpm. The cells were harvested by centrifugation (8000×g, 10 min), washed twice with 0.85 % NaCl, centrifuged again, and then used as the washed cells for the reactions. The cultivation was carried out in test tubes with 10 mL of MRS containing 1 mg/mL of LA, 1 mg/mL of Tween 80 and 5 % (v/v) of the washed cells for 24 h at 37 °C and 120 rpm. Only the strain showing the highest ability to produce CLA was selected for the next steps.

Full paper   Login or join for free to view the full paper.

Reviews

De Man, Rogosa and Sharpe (MRS) Broth from Merck Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Bacterial cell culture media Lactobacillus paracasei using De Man, Rogosa and Sharpe (MRS) Broth from Merck Millipore.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Merck Millipore for De Man, Rogosa and Sharpe (MRS) Broth below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Bacterial cell culture media Lactobacillus paracasei using De Man, Rogosa and Sharpe (MRS) Broth from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms