De Man, Rogosa and Sharpe (MRS) Broth

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-pH at 25 °C pH6.5 ± 0.2. -Incubation Temperature 30–35 °C.

Protocol tips
-pH at 25 °C pH6.5 ± 0.2. -Incubation Temperature 30–35 °C.

Publication protocol

All the lactobacillus strains were activated in MRS broth for 24 h at 37 °C. These strains were cultivated in MRS medium containing 0.06 % LA for 24 h at 28 °C with a shaking rate of 120 rpm. The cells were harvested by centrifugation (8000×g, 10 min), washed twice with 0.85 % NaCl, centrifuged again, and then used as the washed cells for the reactions. The cultivation was carried out in test tubes with 10 mL of MRS containing 1 mg/mL of LA, 1 mg/mL of Tween 80 and 5 % (v/v) of the washed cells for 24 h at 37 °C and 120 rpm. Only the strain showing the highest ability to produce CLA was selected for the next steps.

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Manufacturer protocol

Download the product protocol from Merck Millipore for De Man, Rogosa and Sharpe (MRS) Broth below.

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