TRYPTONE SOYA BROTH (Casein soya bean digest medium) EP/USP/JP/BP

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	For the detection of biofilm formation, S. epidermidis was cultured in TSA medium supplemented with 0.5% glucose. When appropriate, antibiotics were used at the following concentrations: erythromycin (5 μg/ml), spectinomycin (100 μg/ml), chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), and kanamycin (50 μg/ml).

Protocol tips
For the detection of biofilm formation, S. epidermidis was cultured in TSA medium supplemented with 0.5% glucose. When appropriate, antibiotics were used at the following concentrations: erythromycin (5 μg/ml), spectinomycin (100 μg/ml), chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), and kanamycin (50 μg/ml).

Publication protocol

S. epidermidis 1457 (SE1457) and S. aureus RN4220 were kindly provided by Yicun Gao from Hong Kong University; S. epidermidis RP62A (accession number NC_002976) (28) was purchased from the American Type Culture Collection (ATCC; Manassas, VA). All staphylococci were routinely cultured in tryptone soy broth (TSB; Oxoid, Basingstoke, United Kingdom) or tryptone soy agar (TSA). For the detection of biofilm formation, S. epidermidis was cultured in TSA medium supplemented with 0.5% glucose. For the transformation of recombinant plasmids, B2 medium (1% casein hydrolysate, 2.5% yeast extract, 0.5% glucose, 2.5% NaCl, 0.1% K2HPO4 [pH 7.5]) was used for the recovery of staphylococcal cells after electroporation. Luria-Bertani medium was used for culture of Escherichia coli. Oxic conditions were created by incubation into a flask, in which the culture medium did not exceed 15% of the flask volume, and for microaerobic conditions bacteria were incubated into a syringe fully filled with medium. For static incubation under microaerobic conditions, polystyrene plates inoculated with bacteria were placed in an anaerobic bag with Anaerocult C Mini (Merck KGaA, Darmstadt, Germany). When appropriate, antibiotics were used at the following concentrations: erythromycin (5 μg/ml), spectinomycin (100 μg/ml), chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), and kanamycin (50 μg/ml).

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