TRYPTIC SOY BROTH

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	The test tube of 28- day old static cultures (MH strain) was vigorously vortexed for 30 sec, 50 mL were spot deposited on each filter, spread with a spatula and incubated for one day at 37 degrees C

Protocol tips
The test tube of 28- day old static cultures (MH strain) was vigorously vortexed for 30 sec, 50 mL were spot deposited on each filter, spread with a spatula and incubated for one day at 37 degrees C

Publication protocol

15 mL plastic test tubes (polystyrene) were filled with 7 mL trypticase soy broth (Thermo Fisher Scientific Remel Products, Lenexa, KS, USA), inoculated from an overnight agar plate with a single S. epidermidis strain, vortexed for 30 sec and incubated at 37°C without shaking or media change.

Triplicates of test tubes cultures (MH strain) were cultivated for 0, 2, 4, 6, 8, 10, 12, 14, 16 and 18 days as described above. For the CFU determination, the cultures were vortexed for 30 sec, diluted appropriately, plated on tryptic soy agar plates and incubated over night. The CFUs were counted and the median determined.

Sterile Isopore™ membrane filters (0.4 µm, HTTP, Millipore, Ireland) were placed on tryptic soy agar plates (Thermo Fisher Scientific Remel Products, Lenexa, KS, USA). The test tube of 28-day old static cultures (MH strain) was vigorously vortexed for 30 sec, 50 µL were spot deposited on each filter, spread with a spatula and incubated for one day at 37°C.

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