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Animal care followed the recommendations of European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (Council Directive 86/609/EEC) and National Law 1005/92 (rules for protection of experimental animals). Formal approvals to conduct all animal procedures in the experiments have been obtained from the Animal Experimentation Committee of the Biological Research Centre, Hungarian Academy of Sciences (Hungary), and from the local authorities (Permit number: XVI./03835/001/2006). |
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Protocol tips |
Animal care followed the recommendations of European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (Council Directive 86/609/EEC) and National Law 1005/92 (rules for protection of experimental animals). Formal approvals to conduct all animal procedures in the experiments have been obtained from the Animal Experimentation Committee of the Biological Research Centre, Hungarian Academy of Sciences (Hungary), and from the local authorities (Permit number: XVI./03835/001/2006). |
Publication protocol
Animal care followed the recommendations of European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (Council Directive 86/609/EEC) and National Law 1005/92 (rules for protection of experimental animals). Formal approvals to conduct all animal procedures in the experiments have been obtained from the Animal Experimentation Committee of the Biological Research Centre, Hungarian Academy of Sciences (Hungary), and from the local authorities (Permit number: XVI./03835/001/2006).
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Paper title
Exposure to Lipopolysaccharide and/or Unconjugated Bilirubin Impair the Integrity and Function of Brain Microvascular Endothelial Cells
Manufacturer protocol
Download the product protocol from Merck Millipore for DMEM/HAM'S F-12 LIQUID MEDIUM WITHOUT L-GLUTAMINE below.
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