EpiTect Bisulfite Kit

DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A

Experiment
DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A
Product
EpiTect Bisulfite Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Genomic DNA was harvested using phenol-chloroform extraction. 2 μg DNA was treated with bisulfite using EpiTect Bisulfite Kit (Qiagen) and PCR was conducted. Pyrosequencing was then conducted, following the instruction of PyroMark PCR Kit (Qiagen). Three independent experiments were repeated for each sample. Primers for bisulfite sequences in this study were listed in Table ​Table11

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A using EpiTect Bisulfite Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Bisulfite Kit below.

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Videos

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