FITC Annexin V Apoptosis Detection Kit I (RUO)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 4 μl Annexin-V FITC and 4 μl propidium iodide was and incubate for 20 minutes in dark

Protocol tips
Add 4 μl Annexin-V FITC and 4 μl propidium iodide was and incubate for 20 minutes in dark

Publication protocol

Apoptosis assay was performed using a commercially available kit and as per manufacturer’s instruction (BD Biosciences, San Jose, CA, USA). Approximately 0.3 × 106 cells were plated in each well of 6 well plate and left overnight in the incubator. Penfluridol was added in required concentration for 24 h. After 24 h of treatment, cells were harvested by trypsinization and suspended in PBS. Cells were washed and suspended in 150 μl of binding buffer. 4 μl Annexin-V FITC and 4 μl propidium iodide was added and incubated for 20 minutes in dark. Volume was adjusted to 300 μL by adding binding buffer. Samples were analyzed by flow cytometer after vortexing (Accuri C6, Ann Arbor, MI, USA) and as described by us previously29.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I (RUO) below.

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