APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate the cells in the DNA-labeling solution for 60 minutes at 36°C in a temperature controlled bath

Protocol tips
Incubate the cells in the DNA-labeling solution for 60 minutes at 36°C in a temperature controlled bath

Publication protocol

The TUNEL assay was performed according
to the manufacturer’s protocol (A23210; Invitrogen). Briefly,cells were washed with PBS, then fixed with 1% PFA for 15 min on ice, and then incubated with ice-cold 70% ethanol overnight at −20 °C. The cells were washed with wash buffer twice, incubated with DNA-labeling solution containing reaction buffer, TdT enzyme, and 5-Bromo-2′-Deoxyuridine 5′-Triphosphate for 1 h at 36 °C, washed with rinse buffer twice, and then incubated with antibody solution containing AlexaFluor 488 dye labeled
anti-BrdU antibody. The cells were washed three times with rinse buffer and fluorescence was recorded using FLUOStar Omega microplate reader

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - BxPC-3 using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - BxPC-3 using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.