Cell-APOPercentage™ Apoptosis Assay

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	Add dyes and incubate for 30 min, at 37°C/5% CO2

Protocol tips
Add dyes and incubate for 30 min, at 37°C/5% CO2

Publication protocol

RAW 264.7 cells were plated in 24-well tissue culture plates at a cell density of 1 × 105 cells per well. After 24 h, the medium was replaced with medium containing the palmatine compounds. All treatments were performed in triplicate. The cells were treated for 24 h, after which they were harvested and the extent of apoptosis was assessed using the APOPercentage™ assay (Biocolor Ltd., Newtownabbey, Northern Ireland, UK) as previously described [17]. Briefly, the cells were removed by trypsinization, washed with PBS, and stained with APOPercentage dye for 30 min at 37°C. The dye uptake was quantified by the colorimetric method based on the manufacturer's instructions. The cells were lysed with the dye release reagent of attachment, and the absorbance was measured at 550 nm using a Multiskan GO instrument.

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Manufacturer protocol

Download the product protocol from Biocolor for Cell-APOPercentage™ Apoptosis Assay below.

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