RiboPure™ RNA Purification Kit, yeast

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Samples can be flash frozen (-80C) before use, this will likely cause lower RNA yield.		When checking the yield by means of a spectrophotometer, use the supplied TE buffer to zero the machine

Upstream tips
Samples can be flash frozen (-80C) before use, this will likely cause lower RNA yield.
Downstream tips
When checking the yield by means of a spectrophotometer, use the supplied TE buffer to zero the machine

Publication protocol

Total RNA from these cultures was extracted using the RiboPure-Yeast Kit according to manufacturer's instructions (Ambion), starting with a total amount of 3 × 108 cells per sample as recommended for a maximum yield. Total RNA integrity and quantity of the samples were assessed using the Agilent 2100 bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent, see Supplemental Fig. S5A) and NanoDrop 1000 Spectrophotometer (Thermo Scientific). To obtain poly(A)+ RNA, total RNA from yeast was purified by two rounds of selection using the MicroPoly(A)Purist Kit according to manufacturer's instructions (Ambion) to obtain poly(A) RNA from yeast, and the quality of the samples was controlled as above (Supplemental Fig. S5A).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RiboPure™ RNA Purification Kit, yeast below.

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