Anti-LC3B antibody produced in rabbit

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	Western blots were performed to determine the expression levels of LC3, p62 and β-actin. Briefly, AR42J cells were homogenized in T-per buffer (Thermo Fisher) with a protease inhibitor cocktail (Thermo Fisher). Lysates were centrifuged at 16,000 g for 15 min, and then, the samples were boiled in loading buffer before western blot analysis. The protein content was determined by the BCA assay. The western blot was performed according to standard procedures. The protein bands were detected by a ChemiDOC XRS imaging system (Bio-Rad, USA), and β-actin served as a loading control.

Protocol tips

Western blots were performed to determine the expression levels of LC3, p62 and β-actin. Briefly, AR42J cells were homogenized in T-per buffer (Thermo Fisher) with a protease inhibitor cocktail (Thermo Fisher). Lysates were centrifuged at 16,000 g for 15 min, and then, the samples were boiled in loading buffer before western blot analysis. The protein content was determined by the BCA assay. The western blot was performed according to standard procedures. The protein bands were detected by a ChemiDOC XRS imaging system (Bio-Rad, USA), and β-actin served as a loading control.

Publication protocol

Western blots were performed to determine the expression levels of LC3, p62 and β-actin. Briefly, AR42J cells were homogenized in T-per buffer (Thermo Fisher) with a protease inhibitor cocktail (Thermo Fisher). Lysates were centrifuged at 16,000 g for 15 min, and then, the samples were boiled in loading buffer before western blot analysis. The protein content was determined by the BCA assay. The western blot was performed according to standard procedures. The protein bands were detected by a ChemiDOC XRS imaging system (Bio-Rad, USA), and β-actin served as a loading control.

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