STEMdiff™ Pancreatic Progenitor Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Matrix-coated plates should be prepared in advance and brought to room temperature (15 - 25°C) for at least 30 minutes prior to use. - Human ES and iPS cells are ready for passaging when cultures are approximately 70% confluent.	-Thaw entire bottle of Stage 1 Basal Medium at room temperature (15 - 25°C) or overnight at 2 - 8°C. Mix thoroughly. -After preparing the required volumes of media, remaining supplements may be aliquoted and stored at -20°C. Do not exceed the shelf life of the supplements. After thawing aliquoted supplements, use immediately. Do not re-freeze.

Upstream tips
-Matrix-coated plates should be prepared in advance and brought to room temperature (15 - 25°C) for at least 30 minutes prior to use. - Human ES and iPS cells are ready for passaging when cultures are approximately 70% confluent.
Protocol tips
-Thaw entire bottle of Stage 1 Basal Medium at room temperature (15 - 25°C) or overnight at 2 - 8°C. Mix thoroughly. -After preparing the required volumes of media, remaining supplements may be aliquoted and stored at -20°C. Do not exceed the shelf life of the supplements. After thawing aliquoted supplements, use immediately. Do not re-freeze.

Publication protocol

-Thaw entire bottle of Stage 1 Basal Medium at room temperature (15 - 25°C) or overnight at 2 - 8°C. Mix thoroughly.
-After preparing the required volumes of media, remaining supplements may be aliquoted and stored at -20°C. Do not exceed the
shelf life of the supplements. After thawing aliquoted supplements, use immediately. Do not re-freeze.

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Manufacturer protocol

Download the product protocol from STEMCELL technologies for STEMdiff™ Pancreatic Progenitor Kit below.

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