RIPA Lysis Buffer, 10X

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells are lysed using 1 × RIPA lysis buffer supplemented with 1× protease inhibitor (Halt ™ Protease Inhibitor 100×, Thermo Scientific, 78430). - Cell lysates are homogenized mechanically with mortar and pestle and through ice-cold sonication.

Protocol tips
- Cells are lysed using 1 × RIPA lysis buffer supplemented with 1× protease inhibitor (Halt ™ Protease Inhibitor 100×, Thermo Scientific, 78430). - Cell lysates are homogenized mechanically with mortar and pestle and through ice-cold sonication.

Publication protocol

Cells were lysed using 1 × RIPA lysis buffer (EMD Millipore, 20-188) supplemented with 1× protease inhibitor (Halt ™ Protease Inhibitor 100×, Thermo Scientific, 78430). Cell lysates were homogenized mechanically with mortar and pestle and through ice-cold sonication before resolving on 4–20% precast polyacrylamide gels (Bio-Rad, Mini-PROTEAN® TGX™ gels, 456-1094) using 1× Tris/glycine/SDS running buffer (Bio-Rad, 161-0732). Gels were transferred onto nitrocellulose membrane (iBlot® transfer stack, Life Technologies, IB3010-02).

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