Quant-iT™ RiboGreen™ RNA Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit enables quantitation of as little as 1 ng/mL.	Add 1.0 mL of the aqueous working solution of the Quant-iT™ Ribo Green® reagent and incubate for 2 to 5 minutes at room temperature, protected from light..

Upstream tips
This kit enables quantitation of as little as 1 ng/mL.
Protocol tips
Add 1.0 mL of the aqueous working solution of the Quant-iT™ Ribo Green® reagent and incubate for 2 to 5 minutes at room temperature, protected from light..

Publication protocol

Quantitation was calibrated using DNA and RNA standards based on a dilution series of either lambda DNA (Quant-iT PicoGreen dsDNA assay kit; Life Technologies) or rRNA standard from Escherichia coli (Quant-iT RiboGreen RNA assay kit; Life Technologies). Quantitation was performed in triplicate and one batch of standards was used for all measurements in each experiment.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Quant-iT™ RiboGreen™ RNA Assay Kit below.

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