M-PER™ Mammalian Protein Extraction Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cultured cells are washed with cold PBS and lysed on ice in M-PER following the manufacturer's protocol. Except, the lysate is collected and clarified by centrifugation at 12.000g for 10 min at 4°C.

Protocol tips
- Cultured cells are washed with cold PBS and lysed on ice in M-PER following the manufacturer's protocol. Except, the lysate is collected and clarified by centrifugation at 12.000g for 10 min at 4°C.

Publication protocol

Cultured cells were washed with cold PBS and lysed on ice in M-PER (Thermo Scientific, IL). The lysate was collected and clarified by centrifugation at 12.000g for 10 min at 4°C. Total protein was measured in the supernatant by the Lowry method. Electrophoresis of each sample containing 20–30 µg of total protein was performed on a 4–20% LongLife Gel (NuSep, Invitrogen, CA) in Tris Hepes Running Buffer (NuSep, Invitrogen, CA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for M-PER™ Mammalian Protein Extraction Reagent below.

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