RediPlate™ 96 RiboGreen™ RNA Quantitation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Quantitation Range is 3-200 ng	Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Upstream tips
Quantitation Range is 3-200 ng
Protocol tips
Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Publication protocol

Mucosal RNA was extracted from the LCM-dissected jejunum using the RNAqueous-Micro kit (Ambion, Austin, TX) specifically designed to recover small amounts of RNA from LCM samples.

Whole gut RNA was extracted from jejunum collected at different time points following irradiation. Tissues were snap frozen in liquid nitrogen and stored at −80°C. Frozen tissue samples were homogenized in Ultraspec RNA reagent (Biotecx Laboratories, Houston, TX), according to the manufacturer's instructions. Two µg of RNA were kept for microarray analysis (including quality control analysis) and 2 µg were used for real time PCR.

DNase-treated RNA was quantitated using the Rediplate 96 Ribogreen RNA quantitation kit (Invitrogen, Carlsbad, CA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RediPlate™ 96 RiboGreen™ RNA Quantitation Kit below.

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