T-PER™ Tissue Protein Extraction Reagent

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	- Cells were lysed with tissue protein extraction reagent (T-PER) according to the manufacturer’s instructions.

Protocol tips
- Cells were lysed with tissue protein extraction reagent (T-PER) according to the manufacturer’s instructions.

Publication protocol

Cells were lysed with tissue protein extraction reagent (T-PER) (Thermo Scientific; San Jose, CA, USA) according to the manufacturer’s instructions. Protein concentration was measured by the BCA kit (Beyotime, Shanghai, China). Whole cell lysates were electrophoresed on a 10% SDS-PAGE gel and blotted onto a polyvinylidene difluoride membrane. After blocking with 5% BSA in TBS (20 mMTris, 150 mMNaCl, pH 7.4) containing 0.05% Tween 20 (TBST), the membrane was incubated with the antibody of interest (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:200 dilution; Cell Signaling Technology, 1:1000 dilution; Abcam, Cambridge, UK, 1:1000 dilution) and then with peroxidase-conjugated anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:3000 dilution). β-actin (Santa Cruz Biotechnology, 1:2000 dilution) was used as a control. All band intensities were evaluated using an ECL Western blotting kit (Millipore, Belmopán, Belize) and were normalized to those of β-actin. Three independent experiments were performed for each analysis.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for T-PER™ Tissue Protein Extraction Reagent below.

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