RIPA Buffer (10X)

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	- The manufacturer's protocol was followed

Protocol tips
- The manufacturer's protocol was followed

Publication protocol

The whole cell extracts were prepared by lysing cells in RIPA buffer (Cell Signaling, USA). Protein concentration was determined using a BCA Protein Assay Kit (Pierce, USA), and 50 μg of protein was separated by 12.5% SDS-PAGE and transferred onto PVDF membranes. The membranes were then incubated by the PKM2 anti-bodies, and the β-actin expression was evaluated as a control for protein loading.

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