CelLytic™ M

Protocol tips

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	- CellLytic M cell lysis reagent is added to the dishes and kept on ice for 5 min to lyse the cells. - Cells are scrapped and transferred to Eppendorf tubes.

Protocol tips
- CellLytic M cell lysis reagent is added to the dishes and kept on ice for 5 min to lyse the cells. - Cells are scrapped and transferred to Eppendorf tubes.

Publication protocol

200 µl of CellLytic M cell lysis reagent (Sigma-Aldrich, St. Louis, MO, USA) was added to the dishes and kept on ice for 5 min to lyse the cells. The lysed cells were subsequently scraped off and transferred to fresh Eppendorf tubes, then stored at −70°C until blot analysis. The concentration of protein in the lysed sample was analyzed using a bicinchoninic acid assay kit (Thermo Scientific, Pierce, Rockford, IL, USA), after which the samples were heated at 95°C for 5 min, then loaded into SDS-polyacrylamide gels (10%). The separated proteins were transferred to a nitrocellulose membrane (Trans-Blot Turbo™, Hercules, CA, USA), after which the transferred membrane was cut at the desired part and incubated with blocking buffer prior to incubation with various antibodies.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ M below.

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