RIPA Lysis and Extraction Buffer

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	- Cells were lysed in RIPA lysis buffer supplemented with complete protease inhibitor cocktail.

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- Cells were lysed in RIPA lysis buffer supplemented with complete protease inhibitor cocktail.

Publication protocol

HepG2 cells were lysed in RIPA lysis buffer (Thermo Fisher Scientific Inc.) supplemented with complete protease inhibitor cocktail (Roche Diagnostics, Basel, Switzerland). The protein concentrations were determined using a BCA protein assay. Twenty micrograms of whole cell lysate protein was separated by SDS polyacrylamide gel electrophoresis and transferred onto a PVDF membrane. The membranes were blocked with 4% bovine serum albumin and incubated with specific primary antibodies followed by a secondary antibody conjugated to horseradish peroxidase. The blots were then detected by chemiluminescence using Immobilon Western Horseradish Peroxidase Substrate (Millipore), a UVP BioSpectrum AC Imaging System, and VisionWorks LSD Image Acquisition & Analysis Software (UVP LLC, Upland, CA).

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