RIPA Buffer

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Fat tissues are homogenized in 1 mL of RIPA buffer using a homogenizer.

Protocol tips
- Fat tissues are homogenized in 1 mL of RIPA buffer using a homogenizer.

Publication protocol

Murine fat tissues were homogenized in 1 mL of RIPA buffer (10 mM Tris pH 7.8, 1 mM EDTA, 150 mM NaCl, phosphatase inhibitor, and protease inhibitor) using a homogenizer. Tissue lysate was collected and protein analysis was performed using Protein Assay Kit (Bio-Rad, Hercules, CA, USA). Total protein (40 µg) was then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and PVDF membrane transfer (Millipore, Bedford, MA, USA).

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