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3T3-L1 cells were homogenized with the PRO-PREPTM protein extraction solution (Intron Biotechnology Inc., Seongnam, Republic of Korea) and incubated for 10 min on ice to induce cell lysis. The lysates were centrifuged at 11,000× g for 20 min at 4 °C, and the supernatant was transferred to a 1.5-mL microtube.
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Protocol tips |
3T3-L1 cells were homogenized with the PRO-PREPTM protein extraction solution (Intron Biotechnology Inc., Seongnam, Republic of Korea) and incubated for 10 min on ice to induce cell lysis. The lysates were centrifuged at 11,000× g for 20 min at 4 °C, and the supernatant was transferred to a 1.5-mL microtube.
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Publication protocol
3T3-L1 cells were homogenized with the PRO-PREPTM protein extraction solution (Intron Biotechnology Inc., Seongnam, Republic of Korea) and incubated for 10 min on ice to induce cell lysis. The lysates were centrifuged at 11,000× g for 20 min at 4 °C, and the supernatant was transferred to a 1.5-mL microtube.
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