RIPA Buffer

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	After treatment with spilanthol, the cells were lysed in protein lysis buffer (Sigma, St. Louis, MO, USA). Protein samples (10–30 μg) were separated on 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF; Millipore, Billerica, MA, USA).

Protocol tips
After treatment with spilanthol, the cells were lysed in protein lysis buffer (Sigma, St. Louis, MO, USA). Protein samples (10–30 μg) were separated on 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF; Millipore, Billerica, MA, USA).

Publication protocol

After treatment with spilanthol, the cells were lysed in protein lysis buffer (Sigma, St. Louis, MO, USA). Protein samples (10–30 μg) were separated on 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF; Millipore, Billerica, MA, USA).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for RIPA Buffer below.

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