TRI Reagent®

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Total protein is isolated from Brown fat tissues using TRI Reagent following the manufacturer’s instructions.

Protocol tips
- Total protein is isolated from Brown fat tissues using TRI Reagent following the manufacturer’s instructions.

Publication protocol

Total protein was isolated from Brown fat tissues using TRI Reagent (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions. Protein concentration was determined with PierceTM BCA Protein Quantification Assay kit (Thermo-Fisher Scientific, Waltham, MA, USA). Ten micrograms of protein was loaded and separated in a precast 12% gel (Bio-Rad, Hercules, CA, USA) and transferred onto a 0.2 µm nitrocellulose membrane using a Trans-Blot Turbo semi-dry transfer apparatus (Bio-Rad, Hercules, CA, USA). Membranes were blocked for 1 h at room temperature with Odyssey Blocking Buffer (Li-Cor, Lincoln, NE, USA) and incubated overnight at 4 °C with gentle shaking with primary antibodies Membranes were then incubated with the corresponding secondary and the signal was detected using an Odyssey near-infrared scanner (Li-Cor, Lincoln, NE, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Mammalian cells - Mouse_Brown fat using TRI Reagent® from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Mammalian cells - Mouse_Brown fat using TRI Reagent® from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.