Subcellular Protein Fractionation Kit for Cultured Cells

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	Three subcellular fractions (cytoplasmic, membrane, and nuclear) of circumvallate papillae were prepared utilizing the subcellular protein fractionation kit (Thermo Fisher Scientific, Rockford, IL, USA) and characterized by western blotting using fraction-specific protein antibodies.

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Three subcellular fractions (cytoplasmic, membrane, and nuclear) of circumvallate papillae were prepared utilizing the subcellular protein fractionation kit (Thermo Fisher Scientific, Rockford, IL, USA) and characterized by western blotting using fraction-specific protein antibodies.

Publication protocol

Three subcellular fractions (cytoplasmic, membrane, and nuclear) of circumvallate papillae were prepared utilizing the subcellular protein fractionation kit (Thermo Fisher Scientific, Rockford, IL, USA) and characterized by western blotting using fraction-specific protein antibodies.

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