NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

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	Nuclear protein lysates of liver tissue were prepared from rat livers using the nuclear extraction reagent kit (Pierce Biotechnology, USA), according to the manufacturer’s instructions. Whole-protein lysates of liver tissue were extracted using 10% Nonidet P-40 lysis buffer (Invitrogen, Camarillo, CA, USA) supplemented with 1% protease inhibitor cocktail (Sigma-Aldrich), 0.5 mM β-Glycerophosphate (Invitrogen) and 1.0 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich).

Protocol tips

Nuclear protein lysates of liver tissue were prepared from rat livers using the nuclear extraction reagent kit (Pierce Biotechnology, USA), according to the manufacturer’s instructions. Whole-protein lysates of liver tissue were extracted using 10% Nonidet P-40 lysis buffer (Invitrogen, Camarillo, CA, USA) supplemented with 1% protease inhibitor cocktail (Sigma-Aldrich), 0.5 mM β-Glycerophosphate (Invitrogen) and 1.0 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich).

Publication protocol

Nuclear protein lysates of liver tissue were prepared from rat livers using the nuclear extraction reagent kit (Pierce Biotechnology, USA), according to the manufacturer’s instructions. Whole-protein lysates of liver tissue were extracted using 10% Nonidet P-40 lysis buffer (Invitrogen, Camarillo, CA, USA) supplemented with 1% protease inhibitor cocktail (Sigma-Aldrich), 0.5 mM β-Glycerophosphate (Invitrogen) and 1.0 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich).

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