Trichloroacetic acid

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	- Follow the publication protocol.

Protocol tips
- Follow the publication protocol.

Publication protocol

~5×109 spores were resuspended in 200 μl of an extraction buffer (50 mM Tris-HCl, pH 7.4, 8 M urea, and 2% (v/v) 2-mercaptoethanol), heated for 20 min at 90°C, and centrifuged at 13,000× g for 10 min. The supernatant was then treated with 20% (v/v) ice-cold trichloroacetic acid for 30 min on ice and centrifuged at 13,000×g at 4°C for 25 min. The pellet was washed once with 1 ml ice-cold acetone, centrifuged at 7000 r.p.m for 2 min, and dissolved in 100 μl of a solution of 200 mM Tris-HCl (pH 7.4) and 0.1 M glycine.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Trichloroacetic acid below.

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