|- All cells supernatants are centrifuged to remove cellular debris and then subjected to trichloroacetic acid (TCA) precipitation to concentrate the proteins.|
|- Pellets are washed twice with 100% cold acetone.|
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4 years ago
Author: Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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