B-PER™ Bacterial Protein Extraction Reagent
Protein isolation Bacteria - Lactobacillus casei
Protocol tips
| Protocol tips |
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| - Protein isolation was performed following the manufacturer's protocol. |
Publication protocol
After 24 h exposure, all cultures were collected for protein assays. B-PER® Bacterial Protein Extraction Reagent (Pierce®, Rockford, IL) was used to extract the bacterial protein. Bacterial protein concentration was measured on the bacterial extract using a BCA protein assay kit (Pierce®) as per manufacturer’s instructions. Bovine serum albumin was used to calibrate the BCA assay. BCA samples were measured at 560 nm by microplate reader (Magellan™, TECAN, San Jose, CA).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for B-PER™ Bacterial Protein Extraction Reagent below.
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