Cell Counting Kit-8

Protocol tips

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	A549 and PC9 cells transfected with or without siRNA against circABCB10 and a FOXR2 overexpression vector were seeded into 96‐well plates at 5 × 103 cells/well and incubated for increasing durations (0, 12, 24, 48, and 72 hours) before adding 10 μL of Cell Counting Kit‐8 (CCK8) solution per well for 1 hour. Absorbance (450 nm) was measured using a Synergy microplate reader (BioTek, Winooski, VT).

Protocol tips
A549 and PC9 cells transfected with or without siRNA against circABCB10 and a FOXR2 overexpression vector were seeded into 96‐well plates at 5 × 103 cells/well and incubated for increasing durations (0, 12, 24, 48, and 72 hours) before adding 10 μL of Cell Counting Kit‐8 (CCK8) solution per well for 1 hour. Absorbance (450 nm) was measured using a Synergy microplate reader (BioTek, Winooski, VT).

Publication protocol

A549 and PC9 cells transfected with or without siRNA against circABCB10 and a FOXR2 overexpression vector were seeded into 96‐well plates at 5 × 103 cells/well and incubated for increasing durations (0, 12, 24, 48, and 72 hours) before adding 10 μL of Cell Counting Kit‐8 (CCK8) solution per well for 1 hour. Absorbance (450 nm) was measured using a Synergy microplate reader (BioTek, Winooski, VT).

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