Qproteome Bacterial Protein Prep Kit
Protein isolation Bacteria - Salmonella paratyphi A
Protocol tips
| Protocol tips |
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| - Total protein is extracted by following Qproteome Bacterial Protein Prep kit manufacturer's protocol. |
Publication protocol
The cultures were grown until mid-exponential phase (OD600 of 2.7), and the 50 ml volume of cultures were used for a total protein extraction by using Qproteome Bacterial Protein Prep kit (Qiagen).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Papers
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Manufacturer protocol
Download the product protocol from Qiagen for Qproteome Bacterial Protein Prep Kit below.
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