2x Laemmli Sample Buffer
Protein isolation Bacteria - Vibrio alginolyticus
Publication protocol
An equal volume of 2X Laemmli sample buffer (Bio-Rad) was added into each sample and all samples were boiled at 100°C for 5 min and loaded onto a 12% SDS-PAGE. After electrophoresis proteins were transferred to a PVDF membrane (Bio-Rad) and the membrane was blocked with 5% skimmed milk in PBS containing 0.05% (v/v) Tween 20 (PBS-T).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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Manufacturer protocol
Download the product protocol from Bio-Rad Laboratories for 2x Laemmli Sample Buffer below.
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