Human ANGPTL3 (highly sensitive) Assay Kit (27750 )

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Test samples should be measured soon after collection. -For the storage of test samples, store them frozen and do not repeat freeze/thaw cycles. -Thaw the test samples at a low temperature and mix them completely before measurement.	-Dilute the antibody just before the application. -Remove the wash buffer completely by tapping the precoated plate on paper towel. Do not wipe wells with paper towel. -Measurement should be done within 30 minutes after addition of “Stop solution”.	-The remaining “2, Labeled antibody Conc.” should be stored at 4°C in firmly sealed vial.

Upstream tips
-Test samples should be measured soon after collection. -For the storage of test samples, store them frozen and do not repeat freeze/thaw cycles. -Thaw the test samples at a low temperature and mix them completely before measurement.
Protocol tips
-Dilute the antibody just before the application. -Remove the wash buffer completely by tapping the precoated plate on paper towel. Do not wipe wells with paper towel. -Measurement should be done within 30 minutes after addition of “Stop solution”.
Downstream tips
-The remaining “2, Labeled antibody Conc.” should be stored at 4°C in firmly sealed vial.

Publication protocol

Serum specimens were stored at -80°C and thawed twice before assays reported here. In 2017, ANGPTL3, ANGPTL4 and ANGPTL8 protein levels were measured at the Department of Molecular Genetics, Kumamoto University, using a human ANGPTL3 enzyme-linked immune-sorbent assay (ELISA) kit designed to detect full length ANGPTL3 using antibodies respectively targeting N- and C-termini of the protein [Immuno-Biological Laboratories (IBL), Gunma, Japan], and a human ANGPTL4 ELISA kit designed to detect both full length and cleaved protein using antibodies against the N-terminal of ANGPTL4 (IBL). Antibody specificity was confirmed, and none cross-reacted with other ANGPTLs. In particular, antibodies supplied in ANGPTL3 and 4 kits showed little (<0.1%) cross-reactivity with ANGPTL8.

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Manufacturer protocol

Download the product protocol from IBL, Immuno-Biological Laboratories co,Ltd for Human ANGPTL3 (highly sensitive) Assay Kit (27750 ) below.

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Videos

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