Human BDNF PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The concentrations of serum Aβ1-40, cAMP
and BDNF were detected via enzyme-linked immunosorbent assay (ELISA) in strict accordance
with the instructions of the ELISA kit (Wuhan
Boster Biological Technology Co., Ltd., Wuhan,
Hubei, China). The venous blood was drawn from
patients, and centrifuged at 4000× g and 4°C
for 10 min. Then the serum was taken for ELISA. After the standard material was prepared,
it was added together with the sample into the
low-density lipoprotein-coated ELISA plate. The
plate was sealed with the membrane, followed by
incubation at 37°C for 90 min. After the liquid
was poured, the plate was patted dry, and the
biotin-labeled human Aβ1-40 or cAMP or BDNF
antibody was added. Next, the plate was sealed
and incubated at 37°C for 60 min. After the liquid
was poured, the plate was patted dry, washed with
the washing liquid for 3 times, and added with the
avidin-peroxidase complex. The plate was sealed
with the sealing membrane, followed by reaction
at 37°C for 30 min. Next, the developing solution was added for reaction for 30 min, and the stop buffer was used. The optical density (OD)
value of sample in each group was detected at a
wavelength of 450 nm using a microplate reader
(Omega Bio-tek, Norcross, GA, USA). According
to the standard curve, the protein concentration of
sample in each sample was calculated.

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Manufacturer protocol

Download the product protocol from BosterBio for Human BDNF PicoKine™ ELISA Kit below.

Download manufacturer protocol

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