Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All the reagents should be kept at 4oC upon receipt. -Bring all kit components and samples to room temperature (18-25oC) before use.	-Reconstitute the Standard with 1.0mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). -Prepare standard within 15 minutes of performing the assay. If crystals have formed in the Wash Solution concentrate (30×), warm to room temperature and mix gently until the crystals are completely dissolved. -TMB Substrate is easily contaminated. Please protect it from light.	-The reconstituted Standards can be used only once.

Upstream tips
-All the reagents should be kept at 4oC upon receipt. -Bring all kit components and samples to room temperature (18-25oC) before use.
Protocol tips
-Reconstitute the Standard with 1.0mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). -Prepare standard within 15 minutes of performing the assay. If crystals have formed in the Wash Solution concentrate (30×), warm to room temperature and mix gently until the crystals are completely dissolved. -TMB Substrate is easily contaminated. Please protect it from light.
Downstream tips
-The reconstituted Standards can be used only once.

Publication protocol

Breast specimens were collected from 72 patients with breast cancer and 31 healthy controls. The presence of HPV was investigated by polymerase chain reaction (PCR) and genotyping was performed for positive cases. We also evaluated the viral factors such as E6, E2, and E7 in HPV positive cases. Enzyme-linked immunosorbent assay (ELISA (and Real-time PCR techniques were used to measure the expression level of anti-carcinogenic genes, such as p53, retinoblastoma (RB), breast and ovarian cancer susceptibility gene (BRCA1, BRCA2) and inflammatory cytokines, including tumor necrosis factor α (TNF-α), transforming growth factor β (TGF-β), nuclear factor-kB (NF-kB), and different interleukins [ILs] (IL-1,IL6, and IL-17).

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Manufacturer protocol

Download the product protocol from MyBioSource.com for Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit below.

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